Scottish First Minister Nicola Sturgeon appeared to catch the prime minister off-guard on Monday by calling for a new vote on independence before Brexit takes effect.
A war of words over Scottish independence erupted Thursday after Britain's prime minister ruled out an early referendum.
The Prime Minister used her speech to the Conservative Spring Forum in Cardiff to suggest that Ms Sturgeon had been plotting since a year ago to use Brexit as a "pretext" for a new referendum.
Facing two years of what are likely to be hard talks for Britain, May wants to stamp her authority on a new agenda for the country but is struggling to fix the deep divisions exposed by the Brexit vote.
May's position was confirmed by Scottish Conservative leader Ruth Davidson, who said Sturgeon's timetable for a referendum - between autumn 2018 and spring 2019 - would be "rejected conclusively".
"We know that together we are stronger when the nations of our United Kingdom work together rather than split apart". If the concern is timing then she would be open to discussions "within reason", she will say.
Speaking to party activists, the Tory leader said: "It is now clear that using Brexit as the pretext to engineer a second independence referendum has been the SNP's sole objective ever since last June". Politically, it may not be easy for Ms May to refuse another Scottish referendum for which the two Parliaments ~ in Britain and Scotland ~ will have to extend their approval.
Mr Brown, an architect of the 2014 "vow" promising Holyrood more powers in the event of a No vote to independence, will propose a range of controls should be passed to the Scottish Parliament after Brexit.
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"The Scottish Parliament has no power to amend or qualify reserved matters".
Ms Dugdale said: "Gordon and I have been working closely together as Labour puts forward an alternative to the constitutional extremes offered by the SNP and the Tories". Ms Sturgeon said blocking a referendum would be a "democratic outrage".
"It's very clear with the Barroso (doctrine), it would not be a member of the European Union".
"It becomes the will of the democratically elected Parliament of Scotland".
Scotland's First Minister Nicola Sturgeon's plans for a second independence referendum were rejected by May. Such assent is a formality - no monarch has refused to sign a bill for more than 300 years. Putting the national interest above any other consideration.
She says she will do that by March 31, calling it a "decisive moment" for Britain.
"Now is not the time", the British prime minister repeated several times in an interview with ITV News on Thursday.
"Just at this point, all our energies should be focused on our negotiations with the European Union about our future relationship".